Introducing Canada’s state of the art wellness laboratory, FLUIDS iQ, offering tests for food sensitivities (IgG), IBS\IBD, salivary hormones and the revolutionary Zonulin test for intestinal permeability (“Leaky Gut”).
About Zonulin and Intestinal Permeability
Zonulin (Pre-Haptoglobin 2) is a key protein, found in enterocytes, that modulates the permeability of tight junctions between these cells of the wall of the digestive tract1. Zonulin binds to a specific receptor on the surface of the intestinal epithelia and triggers a cascade of biochemical processes that induces paracellular tight junction disassembly and a subsequent permeability increase of the intestinal epithelia, often referred to as “Leaky Gut”. Zonulin is the only physiological modulator of paracellular tight junctions described so far that is involved in controlling the movement of macromolecules and therefore, in the balance of tolerance/immune response.
Celiac Disease (CD):
Gliadin is a wheat glycoprotein which, by a quirk of nature, can activate Zonulin signaling, irrespective of the genetic expression of autoimmunity. The Zonulin effect from gliadin generates a two-way response: Not only can fluid exit, but intestinal contents are able to gain entry in the opposite direction, into the bloodstream.
This Gliadin-Zonulin ‘leaking’ effect is longer and more pronounced (up to 5-fold greater) in the enterocytes of people with celiac disease2.
When intestinal tissue is taken from Celiacs in remission and from non-Celiac controls with digestive complaints, results show that Celiacs may produce 30 times as much Zonulin as non-Celiacs and have a three-fold greater ‘leakiness’ or permeability, even though the non-Celiacs are not eating gluten-free diets while the Celiacs have been off gluten for over two years3. This strongly suggests that something besides gluten may be contributing to ‘Leaky Gut’ in people with CD. It may be that certain types of intestinal dysbiosis (imbalance of bacteria and yeasts in the intestines) prime genetically susceptible individuals to develop CD in response to gluten.
Diabetes Mellitus (DM) – Type 1:
In this auto-immune disease, the level of Zonulin elevation, as well as the resulting increase in intestinal permeability, may precede the clinical manifestation of Diabetes, often by more than a year. Conversely, there is evidence that Type 1 – DM may be avoided by inhibiting the elevation of Zonulin3.
In addition, many people who suffer from CD and DM also suffer from other autoimmune disorders. It has been suggested that increased levels of Zonulin are implicated in the pathogenesis of multiple sclerosis and rheumatoid arthritis4.
FLUIDS iQ is proud to offer an easy to use, dried blood spot (fingerprick) test for Zonulin.
Register online at our website, call, or email us today to apply the cutting edge new Zonulin test for intestinal permeability (“Leaky Gut”) to your practice.
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- Vanuytsel T, Vermeire S and Cleynenet I. The role of Haptoglobin and its related protein, Zonulin, in inflammatory bowel disease. Tissue Barriers. 2013; 1: 1-9.
- Fasano A, Not T, Wang W et al. Zonulin, a newly discovered modulator of intestinal permeability, and its expression in celiac disease. Lancet. 2000; 355: 1518-19.
- Fasano A. Zonulin and its regulation of intestinal barrier function: the biological door to inflammation, autoimmunity, and cancer. Physiol Rev. 2011; 91: 151-175.
- Sapone A et al. Zonulin upregulation is associated with increased gut permeability in subjects with type 1diabetes and their relatives. Diabetes. 2006; 55: 1443-1449.